Accuris PR4005-100 SmartCheck 50bp DNA Ladder, 500ul/100 lanes, 0.1 mg/ml Concentration

The Benchmark Scientific PR4005100 Accuris SmartCheck 50bp DNA Ladder is produced from proprietary plasmids, digested to completion and purified in a multi-step chromatography process. The ladder is supplied in a ready-to-use format that includes loading buffer and tracking dyes. The ladder is supplied at a concentration of 0.1 mg/ml, in a buffer consisting of 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol.
Storage
This product is shipped at ambient temperature. Upon receipt, immediately store at -20° C. Ladders can be stored for up to 15 months at -20° C or at 4° C for up to 6 months.
Quality Control
The ladder is purified by a multi-step chromatography method. Band sharpness is verified by agarose electrophoresis and DNA concentration is verified spectrophotometrically.
General Guidelines
PR4005-100 Accuris SmartCheck DNA ladder is supplied ready-to-use and comes with 1 tube of 500μl, for up to 100 lanes. Briefly vortex the tube and add 5μl to the desired well in the gel. If the ladder is frozen, allow time to thaw and mix thoroughly. The SmartCheck Ladder can be used to approximate the mass of DNA in samples of similar size by comparing the intensity of the sample band to that of the ladder. The masses of each DNA band per 5μl load are given on the back side of this insert.