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Spectrophotometers are advanced analytical instruments used to measure the intensity of light wavelengths absorbed or transmitted by a sample. This capability makes spectrophotometers invaluable in various scientific fields, including chemistry, biology, physics, and materials science, where they are essential for quantitative analysis of substances. The instrument operates by directing light through a sample and measuring the light intensity before and after interaction with the sample, thereby determining the concentration of the substance being analyzed.

Spectrophotometers come in different types, including UV-Vis (ultraviolet-visible) and IR (infrared), each suited to specific types of analysis based on the light wavelength range they utilize. They are widely used for applications such as determining drug concentrations, analyzing environmental samples, and studying biochemical pathways.

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