Whatman 10401116 Protran BA85 Nitrocellulose Blotting Membrane, Circle, 0.45µm Pore Size, 82mm Diameter (Pack of 50)
Protran nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 1% pure nitrocellulose to ensure the highest binding capacity possible.Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. Protran membranes have outstanding handling strength, and are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric, and fluorescent.Unlike PVDF membranes, Protran nitrocellulose does not require a methanol prewetting step. This makes Protran the membrane of choice for proteins, which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
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